However, the field awaits the development of appropriate model animals that recapitulate human pathology ( Wang et al., 2002 Yamasoba et al., 1998 Zheng et al., 1999). In humans, age-related ( Chien and Lin, 2012) and noise-induced hearing loss ( Nelson et al., 2005) are prevalent health problems that require early prevention ( Cunningham and Tucci, 2017). However, multiple structural and cell biological factors influence the actual shape of the tonotopic map ( Temchin and Ruggero, 2014). The basic pattern of the tonotopic map is simple, with higher frequencies on the base of the cochlear spiral and lower frequencies on the apex ( von BГ©kГ©sy and Peake, 1990). The spatial organization of the organ of Corti, the mammalian auditory sensory epithelium, determines the cochlear tonotopic map, which associates the positions of the inner hair cells (IHCs) in the cochlea with local characteristic frequencies.

IntroductionДЄ complete understanding of auditory perception and transduction relies on an accurate reconstruction of the intact, three-dimensional structure of the cochlea. Our method of cellular mapping is effective for system-level phenotyping of the organ of Corti under both physiological and pathological conditions. Application of this method to samples from young, adult, and noise-exposed mice extracted essential information regarding cellular pathology, including longitudinal and radial spatial characteristics of cell loss, implying that multiple mechanisms underlie clustered cell loss. High-fidelity detection and analysis of all hair cell positions along the entire longitudinal axis of the organ of Corti were performed automatically by machine learningвЂ“based pattern recognition. A sorbitol-based optical clearing method enabled imaging of the entire cochlea at subcellular resolution. Here, we report a new analytical pipeline, based on optical clearing of tissue, for the construction of a single-cell resolution map of the organ of Corti.

The highly organized spatial arrangement of sensory hair cells in the organ of Corti is essential for inner ear function.

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